Premium
Nuclear envelope localization of human UNC84A does not require nuclear lamins
Author(s) -
Hasan Sameez,
Güttinger Stephan,
Mühlhäusser Petra,
Anderegg Fabian,
Bürgler Simone,
Kutay Ulrike
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.01.039
Subject(s) - caenorhabditis elegans , lamin , fluorescence recovery after photobleaching , nuclear localization sequence , microbiology and biotechnology , nucleoporin , nuclear pore , rna interference , biology , nuclear protein , inner membrane , rna , nuclear transport , cell nucleus , genetics , nucleus , gene , membrane , transcription factor , mitochondrion
The SUN proteins are a conserved family of proteins in eukaryotes. Human UNC84A (Sun1) is a homolog of Caenorhabditis elegans UNC‐84, a protein involved in nuclear anchorage and migration. We have analyzed targeting of UNC84A to the nuclear envelope (NE) and show that the N‐terminal 300 amino acids are crucial for efficient NE localization of UNC84A whereas the conserved C‐terminal SUN domain is not required. Furthermore, we demonstrate by combining RNA interference with immunofluorescence and fluorescence recovery after photobleaching analysis that localization and anchoring of UNC84A is not dependent on the lamin proteins, in contrast to what had been observed for C. elegans UNC‐84.