Premium
Differential mechanisms of glutamate receptor regulation of SynGAP in cortical neurones
Author(s) -
Rockliffe Nichola,
Gawler Debra
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.100
Subject(s) - kainate receptor , ampa receptor , nmda receptor , glutamate receptor , chemistry , microbiology and biotechnology , receptor , protein subunit , neuroscience , biochemistry , biology , gene
One prime candidate linking N ‐methyl‐ d ‐aspartate (NMDA) receptors to the regulation of the MAP kinase cascade is SynGAP, a negative regulator of Ras. In order to assess how a physiological stimulus can alter SynGAP activity, an appropriate whole cell system must be used and SynGAP must be specifically extracted from membranes whilst preserving the catalytic activity of the protein. Here, we have achieved this and studied the effect of NMDA/α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid (AMPA) and kainate receptor stimulations on SynGAP activity in cortical neurones. Furthermore, we have examined the role of extracellular Ca 2+ , CaM kinase II and the PSD‐95‐NR2B subunit interaction in SynGAP activity regulation and propose a novel convergence of signalling between AMPA, kainate and NMDA receptors.