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LATS2–Ajuba complex regulates γ‐tubulin recruitment to centrosomes and spindle organization during mitosis
Author(s) -
Abe Yoshinori,
Ohsugi Miho,
Haraguchi Keiko,
Fujimoto Jiro,
Yamamoto Tadashi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.096
Subject(s) - centrosome , mitosis , microbiology and biotechnology , biology , multipolar spindles , spindle apparatus , polo like kinase , microtubule , cell cycle , plk1 , cell division , cell , genetics
LATS2 is a human homolog of Drosophila tumor suppressor lats/warts , and encodes a mitotic kinase whose physiological roles remain to be elucidated. We performed yeast two‐hybrid screening and identified a LIM protein Ajuba, as a binding partner of LATS2. LATS2 was localized to the centrosomes throughout the cell cycle and was associated with Ajuba during mitosis, contributing to latter's mitotic phosphorylation. Depletion of LATS2 or Ajuba impaired centrosomal accumulation of γ‐tubulin and spindle formation at the onset of mitosis, suggesting that the LATS2–Ajuba complex regulates organization of the spindle apparatus through recruitment of γ‐tubulin to the centrosome.