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Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF‐κB pathway inhibition
Author(s) -
Nakajima Yumiko,
Furuichi Yasushi,
Biswas Kamal Krishna,
Hashiguchi Teruto,
Kawahara Ko-ichi,
Yamaji Kazuyo,
Uchimura Tomonori,
Izumi Yuichi,
Maruyama Ikuro
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.079
Subject(s) - anandamide , am251 , endocannabinoid system , cannabinoid receptor , chemistry , periodontitis , inflammation , cannabinoid , porphyromonas gingivalis , medicine , endocrinology , downregulation and upregulation , cannabinoid receptor type 2 , receptor , pharmacology , antagonist , biochemistry , gene
Anandamide (AEA) exhibits anti‐inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro‐inflammatory mediators (IL‐6, IL‐8 and MCP‐1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS‐triggered NF‐κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.