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Transcription factor cycling on the insulin promoter
Author(s) -
Barrow John,
Hay Colin W.,
Ferguson Laura A.,
Docherty Hilary M.,
Docherty Kevin
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.061
Subject(s) - chromatin immunoprecipitation , insulin , transcription factor , promoter , immunoprecipitation , transcription (linguistics) , chemistry , insulin receptor , binding site , medicine , biology , endocrinology , microbiology and biotechnology , biochemistry , gene , insulin resistance , gene expression , linguistics , philosophy
Using MIN6 β‐cells and chromatin immunoprecipitation (ChIP) assays, the chronological sequence of binding of MafA, E47/β2 and PDX‐1 to the insulin promoter in living β‐cells were investigated. All four factors were shown to bind to the mouse insulin 2 promoter in a cyclical manner with a periodicity of approximately 10–15 min. The cyclical binding of MafA, E47 and β2 was largely unaffected by the glucose or insulin concentration in the media. However, the binding and cycling of PDX‐1 was markedly abolished in low glucose (1 mM), and this was reversed in the presence of low concentrations of insulin.