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Elasticity and adhesion of resting and lipopolysaccharide‐stimulated macrophages
Author(s) -
Leporatti Stefano,
Gerth Anja,
Köhler Guido,
Kohlstrunk Bernd,
Hauschildt Sunna,
Donath Edwin
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.037
Subject(s) - lipopolysaccharide , elasticity (physics) , viscoelasticity , biophysics , chemistry , adhesion , stimulation , cytoskeleton , membrane , materials science , cell , immunology , composite material , biology , biochemistry , endocrinology , organic chemistry
Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS‐stimulated cells (separation force = 37 ± 3 nN) was almost twice as high as that of resting macrophages (16 ± 1 nN). Upon retraction pulling of membrane tethers was observed. Tether lengths and forces at which rupture take place did not depend on stimulation. The reduced Young's modulus K , a measure of cytoskeleton elasticity, was three times lower than that of the control. The data show that LPS has profound effects on cytomechanical and adhesion properties of macrophages.