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Thr 199 phosphorylation targets nucleophosmin to nuclear speckles and represses pre‐mRNA processing
Author(s) -
Tarapore Pheruza,
Shinmura Kazuya,
Suzuki Hitoshi,
Tokuyama Yukari,
Kim Song-Hee,
Mayeda Akila,
Fukasawa Kenji
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.022
Subject(s) - nucleophosmin , cyclin dependent kinase 2 , cyclin dependent kinase 1 , phosphorylation , casein kinase 1 , microbiology and biotechnology , biology , kinase , protein kinase a , biochemistry , cell cycle , gene
Nucleophosmin (NPM) is a multifunctional phosphoprotein, being involved in ribosome assembly, pre‐ribosomal RNA processing, DNA duplication, nucleocytoplasmic protein trafficking, and centrosome duplication. NPM is phosphorylated by several kinases, including nuclear kinase II, casein kinase 2, Polo‐like kinase 1 and cyclin‐dependent kinases (CDK1 and 2), and these phosphorylations modulate the activity and function of NPM. We have previously identified Thr 199 as the major phosphorylation site of NPM mediated by CDK2/cyclin E (and A), and this phosphorylation is involved in the regulation of centrosome duplication. In this study, we further examined the effect of CDK2‐mediated phosphorylation of NPM by using the antibody that specifically recognizes NPM phosphorylated on Thr 199 . We found that the phospho‐Thr 199 NPM localized to dynamic sub‐nuclear structures known as nuclear speckles, which are believed to be the sites of storage and/or assembly of pre‐mRNA splicing factors. Phosphorylation on Thr 199 by CDK2/cyclin E (and A) targets NPM to nuclear speckles, and enhances the RNA‐binding activity of NPM. Moreover, phospho‐Thr 199 NPM, but not unphosphorylated NPM, effectively represses pre‐mRNA splicing. These findings indicate the involvement of NPM in the regulation of pre‐mRNA processing, and its activity is controlled by CDK2‐mediated phosphorylation on Thr 199 .