Premium
NF‐κB activation is required for cisplatin‐induced apoptosis in head and neck squamous carcinoma cells
Author(s) -
Kim Seong Bum,
Kim Jong Soo,
Lee Ji Hye,
Yoon Won Joon,
Lee Dong Seuk,
Ko Myoung Seok,
Kwon Byung Suk,
Choi Dae Hwa,
Cho Hong Rae,
Lee Byung Ju,
Chung Dae Kyun,
Lee Hyeon Woo,
Park Jeong Woo
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.012
Subject(s) - cisplatin , apoptosis , cancer research , head and neck squamous cell carcinoma , head and neck , medicine , squamous carcinoma , head and neck cancer , nf κb , carcinoma , basal cell , oncology , chemistry , chemotherapy , cancer , surgery , biochemistry
This study demonstrates a requirement for NF‐κB activation in cis ‐diamminedichloroplatinum (cisplatin)‐induced apoptosis in human head and neck squamous cell carcinoma (HNSCC) cell lines. This conclusion was supported by the following observations: cisplatin induced IκBα degradation and NF‐κB‐dependent transcriptional activation prior to cell death; pyrrolidine dithiocarbamate (PDTC), a chemical inhibitor of NF‐κB activation, prevented apoptosis; lactacystin, an inhibitor of IκBα degradation, also prevented apoptosis; and finally, the expression of a super‐repressor mutant IκBα blocked apoptosis. The expression of tumor necrosis factor α (TNFα) was promoted by cisplatin treatment and was suppressed by PDTC treatment. In addition, a neutralizing antibody against TNFα protected cells from cisplatin‐induced apoptosis. These findings suggest that NF‐κB activation is required for cisplatin‐induced apoptosis and TNFα may play an important role in NF‐κB‐mediated apoptosis in cisplatin‐treated HNSCC cell lines.