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Expression of yeast transcriptional activator MSN1 promotes accumulation of chromium and sulfur by enhancing sulfate transporter level in plants
Author(s) -
Kim Young Jin,
Kim Jong Hoon,
Lee Chang Eun,
Mok Young Geun,
Choi Jong Soon,
Shin Hyoung Sun,
Hwang Seongbin
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.001
Subject(s) - yeast , nicotiana tabacum , saccharomyces cerevisiae , transporter , activator (genetics) , sulfate , transgene , sulfur , chromium , biochemistry , chemistry , gene expression , biology , gene , organic chemistry
MSN1 is a putative yeast transcriptional activator involved in chromium (Cr) accumulation. Here we show that overexpression of MSN1 enhances Cr and sulfur accumulation and Cr tolerance in transgenic tobacco. In addition, we found that expression of NtST1 ( Nicotiana tabacum sulfate transporter 1) was elevated in MSN1‐ expressing transgenic tobacco, suggesting that chromate and sulfate are taken up via the sulfate transporter in plants. Supporting this, expression of NtST1 increased levels of Cr and S in Saccharomyces cerevisiae . Our findings suggest that yeast transcriptional activators can be used for developing effective metal remediators, and for improving the nutritional status of plants.