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Transformation‐associated gene regulation by ATF6α during hepatocarcinogenesis
Author(s) -
Arai Masaaki,
Kondoh Nobuo,
Imazeki Nobuo,
Hada Akiyuki,
Hatsuse Kazuo,
Kimura Fumihiro,
Matsubara Osamu,
Mori Kazutoshi,
Wakatsuki Toru,
Yamamoto Mikio
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.11.072
Subject(s) - atf6 , unfolded protein response , xbp1 , endoplasmic reticulum , biology , activating transcription factor , thapsigargin , microbiology and biotechnology , downregulation and upregulation , gene , transcription factor , regulation of gene expression , gene expression , transmembrane protein , atf4 , transfection , cancer research , genetics , receptor , rna splicing , rna
We have previously reported that the endoplasmic reticulum (ER) stress‐regulated transmembrane transcription factor 6 α (ATF6α) is implicated in the pathogenesis of hepatocellular carcinomas (HCCs). In order to further identify genes that are regulated by ATF6 α, the global gene expression profiles of the ATF6α ‐transfected and untransfected HCC cell line, HLF, were analyzed. These results were then compared with the differential gene expression patterns of poorly differentiated HCC and control non‐tumorous liver tissue. Our findings demonstrate that at least 18 genes are specifically upregulated by ATF6α , while another UPR mediator, XBP1 or ER‐stress inducer, thapsigargin could partially stimulate or even repress some of them in HCC cells. Moreover, six of these identified genes contain potential ER stress‐responsive elements and/or unfolded protein response elements in their 5′ regulatory regions.