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Osmotic stress sensing in Populus : Components identification of a phosphorelay system
Author(s) -
Chefdor Françoise,
Bénédetti Hélène,
Depierreux Christiane,
Delmotte Francis,
Morabito Domenico,
Carpin Sabine
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.11.051
Subject(s) - osmotic shock , histidine , biochemistry , biology , complementary dna , yeast , kinase , microbiology and biotechnology , gene , amino acid
To study the Populus response to an osmotic stress, we have isolated one cDNA encoding a histidine‐aspartate kinase (HK1) and four cDNAs encoding histidine‐containing phosphotransfer proteins (HPts), HPt1–4. The predicted HK1 protein shares a typical structure with ATHK1 and SLN1 osmosensors. The 4 HPTs are characterized by the histidine phosphotransfer domain. We have shown that HK1 is upregulated during an osmotic stress in hydroponic culture. We have detected an interaction between HK1 and HPt2, using the yeast two‐hybrid system. These results suggest the existence of a multi‐step phosphorelay pathway probably involved in osmotic stress sensing in Populus .