Isolated ε subunit of  Bacillus subtilis  F 1 ‐ATPase binds ATP | Zendy

Kato-Yamada Yasuyuki | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Isolated ε subunit of Bacillus subtilis F 1 ‐ATPase binds ATP

Author(s) -

Kato-Yamada Yasuyuki

Publication year - 2005

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2005.11.036

Subject(s) - bacillus subtilis , atpase , protein subunit , chemistry , adenosine triphosphate , biochemistry , v atpase , enzyme , biophysics , microbiology and biotechnology , biology , bacteria , genetics , gene

Previously, we demonstrated ATP binding to the isolated ε subunit of F 1 ‐ATPase from thermophilic Bacillus PS3 [Kato‐Yamada Y., Yoshida M. (2003) J. Biol. Chem. 278, 36013]. However, whether it is a general feature of the ε subunit from other sources is yet unclear. Here, using a sensitive method to detect weak interactions between fluorescently labeled ε subunit and nucleotide, it was shown that the ε subunit of F 1 ‐ATPase from Bacillus subtilis also bound ATP. The dissociation constant for ATP binding at room temperature was calculated to be 2 mM, which may be suitable for sensing cellular ATP concentration in vivo.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research