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Modeling the highly specific ribotoxin recognition of ribosomes
Author(s) -
García-Mayoral Flor,
García-Ortega Lucía,
Álvarez-García Elisa,
Bruix Marta,
Gavilanes José G.,
Martínez del Pozo Álvaro
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.11.027
Subject(s) - ribosome , ribosomal rna , ricin , ribosomal protein , ribosome inactivating protein , chemistry , oligonucleotide , nucleotide , biochemistry , biology , rna , dna , gene , toxin
The three‐dimensional structures of the α‐sarcin ribotoxin and its Δ(7–22) deletion mutant, both complexed with a 20‐mer oligonucleotide mimicking the sarcin/ricin loop (SRL) of the ribosome, have been docked into the structure of the Halobacterium marismortui ribosome by fitting the nucleotide atomic coordinates into those of the ribosomal SRL. This study has revealed that two regions of the ribotoxin, residues 11–16 and 84–85, contact the ribosomal proteins L14 (residues 99–105) and L6 (residues 88–92), respectively. The first of these two ribotoxin regions appears to be crucial for its specific ribosome recognition.