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The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
Author(s) -
Geng Hua,
Liu Yuet-Man,
Chan Wai-Sing,
Lo Anthony Wing-Ip,
Au Deborah Mun-Yee,
Waye Mary Miu-Yee,
Ho Yuan-Yuan
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.11.007
Subject(s) - coronavirus , respiratory system , covid-19 , medicine , biology , virology , pathology , disease , infectious disease (medical specialty)
The SARS‐CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS‐CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.