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Polyomavirus EGFP‐pseudocapsids: Analysis of model particles for introduction of proteins and peptides into mammalian cells
Author(s) -
Bouřa E.,
Liebl D.,
Špíšek R.,
Frič J.,
Marek M.,
Štokrová J.,
Holáň V.,
Forstová J.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.10.062
Subject(s) - green fluorescent protein , in vitro , microbiology and biotechnology , secretion , downregulation and upregulation , capsid , proteasome , transfection , biology , chemistry , cell culture , gene , biochemistry , genetics
A vector for preparation of mouse polyomavirus capsid‐like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C‐terminal part of the VP3 minor protein (EGFP‐VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP‐VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co‐stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion.