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RFX‐1, a putative alpha Adducin interacting protein in a human kidney library
Author(s) -
Boito Rosalia,
Menniti Miranda,
Amato Rosario,
Palmieri Camillo,
Marinaro Cinzia,
Iuliano Rodolfo,
Tripodi Grazia,
Cusi Daniele,
Fuiano Giorgio,
Perrotti Nicola
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.10.040
Subject(s) - colocalization , immunoprecipitation , microbiology and biotechnology , alpha (finance) , biology , chemistry , gene , biochemistry , medicine , construct validity , nursing , patient satisfaction
Adducin regulates tubular absorption of sodium by modulating the expression levels of the sodium–potassium‐ATPase in renal tubular cells. Adducin is a candidate gene in the pathogenesis of hypertension. Yeast two hybrid screen showed a specific interaction between human alpha Adducin and the regulatory factor for X box (RFX‐1), a nuclear protein that down regulates the expression of several proteins in non neuronal cells. The interaction was confirmed in cells through coimmunoprecipitation and colocalization experiments. The binding of alpha Adducin to RFX‐I and their nuclear co‐localization suggests that Adducin can have a role in modulating the transcriptional regulating activity of RFX‐I.