Premium
Platelet‐activating factor induces matrix metalloproteinase‐9 expression through Ca 2+ ‐ or PI3K‐dependent signaling pathway in a human vascular endothelial cell line
Author(s) -
Ko Hyun-Mi,
Kang Jee-Hae,
Choi Jung-Hwa,
Park Sung Jun,
Bai Suk,
Im Suhn-Young
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.10.027
Subject(s) - signal transduction , mapk/erk pathway , pi3k/akt/mtor pathway , microbiology and biotechnology , phosphatidylinositol , angiogenesis , chemistry , kinase , platelet activating factor , biology , cancer research , endocrinology
Platelet‐activating factor (PAF) augments angiogenesis by promoting the synthesis of a variety of angiogenic factors, via the nuclear factor (NF)‐κB activation. Recently, we reported that PAF upregulates MMP‐9 expression in a NF‐κB‐dependent manner. In this study, we investigated the signaling pathway involved in PAF‐induced MMP‐9 expression in ECV304 cells. Our current data indicate that the Ca 2+ ‐ or phosphatidylinositol 3‐kinase (PI3K)‐dependent signaling pathway is necessary for PAF‐induced MMP‐9 expression. Furthermore, PAF‐induced NF‐κB activation was blocked by selective inhibitors of Ca 2+ , PI3K, or extracellular signal‐regulated kinase (ERK). Our results suggest that PAF‐induced MMP‐9 expression, in a NF‐κB‐dependent manner, is regulated by Ca 2+ , PI3K and ERK signaling pathways.