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CD45 expression on rat acinar cells: Involvement in pro‐inflammatory cytokine production
Author(s) -
De Dios Isabel,
Ramudo Laura,
Alonso José R.,
Recio Javier S.,
Garcia-Montero Andrés C.,
Manso Manuel A.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.10.017
Subject(s) - cytokine , microbiology and biotechnology , chemistry , regulator , acinar cell , biology , endocrinology , medicine , immunology , pancreas , gene , biochemistry
CD45 transduces activation signals in inflammatory cells. We investigate CD45 expression on pancreatic acinar cells and examine its role in the inflammatory response which these cells have also shown under certain circumstances. Similar CD45 mRNA levels were found in acinar cells and leukocytes (positive control). Flowcytometric and immunohistochemical analysis showed a heterogeneous CD45 distribution on acinar cells. Activation of acinar cells by incubation with pancreatitis‐associated ascitic fluid as evidencied by TNF‐〈alpha〉 production resulted in a decreased CD45 expression, suggesting that CD45 acts as a negative regulator of cytokine production. As a validation of this finding in vivo, a decrease in the acinar CD45 expression in parallel with an increased ability to produce TNF‐〈alpha〉 was found in rats with acute pancreatitis. Our data show that CD45 is constitutively expressed in acinar cells and suggest that it plays an important role in negatively regulating cytokine production.