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The PDZ/coiled‐coil domain containing protein PIST modulates insulin secretion in MIN6 insulinoma cells by interacting with somatostatin receptor subtype 5
Author(s) -
Wente Wolf,
Efanov Alexander M.,
Treinies Iris,
Zitzer Heike,
Gromada Jesper,
Richter Dietmar,
Kreienkamp Hans-Jürgen
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.10.009
Subject(s) - internalization , somatostatin , insulinoma , somatostatin receptor , secretion , microbiology and biotechnology , somatostatin receptor 1 , chemistry , receptor , insulin , somatostatin receptor 2 , biology , medicine , endocrinology , biochemistry
The multi‐domain protein PIST (protein interacting specifically with Tc10) interacts with the SSTR5 (somatostatin receptor 5) and is responsible for its intracellular localization. Here, we show that PIST is expressed in pancreatic β‐cells and interacts with SSTR5 in these cells. PIST expression in MIN6 insulinoma cells is reduced by somatostatin (SST). After stimulation with SST, SSTR5 undergoes internalization together with PIST. MIN6 cells over‐expressing PIST display enhanced glucose‐stimulated insulin secretion and a decreased sensitivity to SST‐induced inhibition of insulin secretion. These data suggest that PIST plays an important role in insulin secretion by regulating SSTR5 availability at the plasma membrane.