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The “resurrection method” for modification of specific proteins in higher plants
Author(s) -
Mori Masashi,
Dohi Koji
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.10.005
Subject(s) - nicotiana benthamiana , gene , gene silencing , genetically modified crops , biology , messenger rna , gene expression , transgene , sequence (biology) , genetics , rna , codon usage bias , microbiology and biotechnology , genome
We describe a new method designated “the resurrection method” by which a modified protein is expressed in higher plants in place of the original protein. The modified gene constructed by introducing synonymous codon substitutions throughout the original gene to prevent the sequence‐specific degradation of its mRNA during RNA silencing is expressed while the expression of the original gene is suppressed. Here, we report the successful alteration of the biochemical properties of green fluorescent protein expressed in transgenic Nicotiana benthamiana , suggesting that this method could be useful for gene control in living plants.