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IL‐4 modulates the histamine content of mast cells in a mast cell/fibroblast co‐culture through a Stat6 signaling pathway in fibroblasts
Author(s) -
Nabeshima Yukiko,
Hiragun Takaaki,
Morita Eishin,
Mihara Shoji,
Kameyoshi Yoshikazu,
Hide Michihiro
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.09.104
Subject(s) - histamine , mast cell , fibroblast , interleukin 33 , microbiology and biotechnology , interleukin 5 , cell culture , immunoglobulin e , in vitro , biology , chemistry , immunology , interleukin , cytokine , antibody , endocrinology , biochemistry , genetics
IL‐4 plays a crucial role in the pathogenesis of allergic diseases, such as the induction of IgE synthesis and the development of mast cells. To further understand the effect of IL‐4 on mast cells in skin, we utilized a mast cell/fibroblast co‐culture system as an in vitro model of dermal mast cells. IL‐4 induced mast cell growth in the culture with fibroblasts. Immunoblot analysis revealed that IL‐4 activated Stat6 in both mast cells and fibroblasts. The over‐expression of dominant‐negative Stat6 in fibroblasts in the presence of IL‐4 decreased the histamine content per mast cell, but not the number of mast cells. In contrast, the over‐expression of constitutively‐active Stat6 in fibroblasts increased the histamine content per mast cell, indicating that the activation of Stat6 in fibroblasts supports the maturation of mast cells co‐cultured with fibroblasts.