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Claudins upregulation in human colorectal cancer
Author(s) -
de Oliveira Silvia Souza,
de Oliveira Ivanir Martins,
De Souza Wanderley,
Morgado-Díaz José Andrés
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.09.091
Subject(s) - claudin , tight junction , paracellular transport , colorectal cancer , downregulation and upregulation , barrier function , occludin , cancer research , epithelium , cancer , adenocarcinoma , biology , chemistry , pathology , microbiology and biotechnology , medicine , permeability (electromagnetism) , gene , biochemistry , membrane
In colorectal cancer tight junction molecular and morphological alterations are poorly understood. In this study, adenocarcinoma tissues and their paired normal mucosa ( n = 12) were analyzed for tight junction alterations molecular. The expression of claudin‐1, ‐3 and ‐4 was upregulated 5.7‐, 1.5‐ and 2.4‐fold, respectively, in colorectal tumor tissues in comparison to the normal ones. Although tight junction remains in the cancerous epithelium, its barrier function was altered. Despite claudins overexpression, paracellular permeability to ruthenium red was increased and a significant disorganization of tight junction strands was observed in freeze fracture replicas. Whereas the functional significance of claudin overexpression in colorectal cancer is unclear, these proteins can become potential markers and targets in colorectal cancer.
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