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Developmental shift in the apostat: Comparison of neurones and astrocytes
Author(s) -
Stoka Veronika,
Chen Sylvia F.,
Turk Vito,
Bredesen Dale E.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.09.087
Subject(s) - astrocyte , downregulation and upregulation , incubation , apoptosis , microbiology and biotechnology , chemistry , cytochrome c , biology , neuroscience , biochemistry , central nervous system , gene
The intrinsic pathway of apoptosis was investigated in cell‐free extracts of neurones and astrocytes at various stages of maturation. Neuronal extracts were activated 65‐fold after 3 days, 9‐fold after 7 days, and were not activated after 10 days in culture. In contrast, astrocyte extracts were activated to a similar extent at all stages, up to 60 days in culture. The co‐incubation of neuronal and astrocyte extracts followed by addition of cytochrome c /2′‐deoxyadenosine 5′‐triphosphate led to a 40‐fold activation, suggesting that the development‐associated neuronal shift does not involve the appearance of a dominant inhibitor, but rather downregulation of some key component(s) involved in caspase activation.