CCAAT/enhancer binding protein‐β (C/EBP‐β), a pivotal regulator of the TATA‐less promoter in the rat catalase gene | Zendy

Taniguchi Makoto | Zendy; Hashimoto Mayumi | Zendy; Hori Naohiro | Zendy; Sato Kenzo | Zendy

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CCAAT/enhancer binding protein‐β (C/EBP‐β), a pivotal regulator of the TATA‐less promoter in the rat catalase gene

Author(s) -

Taniguchi Makoto,

Hashimoto Mayumi,

Hori Naohiro,

Sato Kenzo

Publication year - 2005

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2005.09.068

Subject(s) - tata box , microbiology and biotechnology , promoter , enhancer , transcription (linguistics) , response element , ccaat enhancer binding proteins , transcription factor , biology , transcriptional regulation , gene , gene isoform , chemistry , gene expression , dna binding protein , biochemistry , linguistics , philosophy

The rat catalase gene carries a TATA‐less promoter and its transcriptional mechanism is interesting because of downregulation in liver injury. We characterized the core element in the promoter and found that C/EBP‐β binding downstream of the transcription initiation site plays a crucial role for transcription. The multiple complexes binding to the promoter were composed of homodimers and heterodimers of C/EBP‐β isoforms. Transduction of the C/EBP‐β gene showed complete reconstitution of multiple binding complexes in HeLa cells, similar to normal liver. Furthermore, C/EBP‐β was observed to bind to the endogenous catalase promoter. These data suggest that multiple complex formation of C/EBP‐β regulates transcription in the TATA‐less catalase promoter.

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