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Expression of macrophage‐selective markers in human and rodent adipocytes
Author(s) -
Khazen Wael,
M'Bika Jean-Pierre,
Tomkiewicz Céline,
Benelli Chantal,
Chany Charles,
Achour Ammar,
Forest Claude
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.09.032
Subject(s) - cd68 , macrophage , cd14 , adipose tissue , biology , adipose tissue macrophages , microbiology and biotechnology , cell culture , immunology , immunohistochemistry , white adipose tissue , flow cytometry , endocrinology , biochemistry , in vitro , genetics
CD14, CD68 and/or mouse F4/80 or human epidermal growth factor module‐containing mucin‐like receptor 1 (EMR1) are widely used as macrophage‐specific markers. Since macrophages infiltrate several tissues during inflammatory processes, CD14, CD68 and EMR1‐F4/80 have been employed to discriminate between tissue‐containing macrophages, like adipose tissue (AT), and other cells. Using real‐time PCR experiments, we show that isolated adipocytes from humans and mice AT express high levels of CD14 and CD68 mRNA, whereas EMR1‐F4/80 is mainly present in the macrophage‐containing stroma‐vascular fraction. Furthermore, fibroblasts‐like cells (adipoblasts), preadipocytes and adipocytes from the murine cell lines, 3T3‐F442A and BFC‐1, express CD14 and CD68 mRNA and protein as determined by fluorescence‐activated cell sorter, but not F4/80 which, as expected, is strongly expressed in the macrophage cell line RAW264.7. These results reinforce the view that EMR1‐F4/80 is the best macrophage marker to date and show that CD14 and CD68 are not macrophage‐specific proteins.