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Identification of a novel bean α‐amylase inhibitor with chitinolytic activity
Author(s) -
Dayler Charles S.A.,
Mendes Paulo A.M.,
Prates Maura V.,
Bloch Carlos,
Franco Octavio L.,
Grossi-de-Sá Maria F.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.09.030
Subject(s) - phaseolus , amylase , pest analysis , enzyme , glycoside hydrolase , biology , starch , biochemistry , enzyme assay , chemistry , botany
Zabrotes subfasciatus is a devastating starch‐dependent storage bean pest. In this study, we attempted to identify novel α‐amylase inhibitors from wild bean seeds, with efficiency toward pest α‐amylases. An inhibitor named Phaseolus vulgaris chitinolytic α‐amylase inhibitor (PvCAI) was purified and mass spectrometry analyses showed a protein with 33 330 Da with the ability to form dimers. Purified PvCAI showed significant inhibitory activity against larval Z. subfasciatus α‐amylases with no activity against mammalian enzymes. N‐terminal sequence analyses showed an unexpected high identity to plant chitinases from the glycoside hydrolase family 18. Furthermore, their chitinolytic activity was also detected. Our data provides compelling evidence that PvCAI also possessed chitinolytic activity, indicating the emergence of a novel α‐amylase inhibitor class.