Premium
Compact oleic acid in HAMLET
Author(s) -
Fast Jonas,
Mossberg Ann-Kristin,
Nilsson Hanna,
Svanborg Catharina,
Akke Mikael,
Linse Sara
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.089
Subject(s) - hamlet (protein complex) , oleic acid , heteronuclear molecule , nuclear magnetic resonance spectroscopy , chemistry , lactalbumin , two dimensional nuclear magnetic resonance spectroscopy , fatty acid , alpha lactalbumin , crystallography , nuclear overhauser effect , spectroscopy , nuclear magnetic resonance , biophysics , stereochemistry , biochemistry , biology , physics , genetics , quantum mechanics
HAMLET (human alpha‐lactalbumin made lethal to tumor cells) is a complex between α‐lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13 C‐oleic acid in HAMLET, and to study the 15 N‐labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule‐like complex under physiological conditions.