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Identification and analysis of Hic‐5/ARA55 isoforms: Implications for integrin signaling and steroid hormone action
Author(s) -
Gao Zhengliang,
Schwartz Lawrence M.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.086
Subject(s) - gene isoform , alternative splicing , biology , microbiology and biotechnology , computational biology , genetics , gene
Hic‐5/ARA55 is a LIM‐only member of the paxillin superfamily. Conflicting reports have suggested that Hic‐5/ARA55 can both repress and enhance a number of biological processes, including myogenesis and tumorigenesis. With two Hic‐5 isoforms documented, we hypothesized that multiple Hic‐5 isoforms may exist that have both overlapping and isoform‐specific functions. To test this hypothesis, we performed an extensive analysis of Hic‐5 transcripts in both cell lines and mouse tissues and found 12 distinct isoforms that fall into two sub‐families. These isoforms are derived from both alternative splicing and alternative transcriptional start sites (TSS). Hic‐5 expression is regulated in a temporally and spatially controlled manner in vivo. The identification of numerous Hic‐5 isoforms suggests that Hic‐5 subsumes a number of distinct roles in cells and may explain the range of biological responses attributed to Hic‐5.