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Identification of plasma membrane associated mature β‐hexosaminidase A, active towards GM2 ganglioside, in human fibroblasts
Author(s) -
Mencarelli Simona,
Cavalieri Cristina,
Magini Alessandro,
Tancini Brunella,
Basso Luisa,
Lemansky Peter,
Hasilik Andrej,
Li Yu-Teh,
Chigorno Vanna,
Orlacchio Aldo,
Emiliani Carla,
Sonnino Sandro
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.081
Subject(s) - biotinylation , immunocytochemistry , ganglioside , hexosaminidase , biochemistry , membrane , enzyme , chemistry , cell membrane , microbiology and biotechnology , biology , endocrinology
Mature β‐hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of β‐hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated β‐hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin.