Premium
Glucose deprivation mediates interaction between CTDK‐I and Snf1 in Saccharomyces cerevisiae
Author(s) -
Van Driessche Benoit,
Coddens Séverine,
Van Mullem Vincent,
Vandenhaute Jean
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.057
Subject(s) - saccharomyces cerevisiae , mutant , kinase , ask1 , biochemistry , microbiology and biotechnology , protein kinase a , gene , biology , chemistry , mitogen activated protein kinase kinase
Ctk1 is a kinase involved in transcriptional control. We show in the two‐hybrid system that Ctk1 interacts with Snf1, a kinase regulating glucose‐dependent genes. Co‐purification experiments confirmed the two‐hybrid interaction but only when cells were grown at low glucose concentrations. Deletion of Ctk1 or its associated partners, Ctk2 and Ctk3, conferred synthetic lethality with null mutants of Snf1 or Snf1‐associated proteins. Northern blot analysis suggested that Ctk1 and Snf1 act together in vivo to regulate GSY2 . These findings support the view that Ctk1 interacts with Snf1 in a functional module involved in the cellular response to glucose limitation.