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The polycomb group gene product Mel‐18 interacts with cyclin D2 and modulates its activity
Author(s) -
Chun Taehoon,
Rho Seung Bae,
Byun Hyun-Jung,
Lee Jung-Yeon,
Kong Gu
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.050
Subject(s) - cyclin a2 , cyclin d2 , cyclin , cyclin a , cyclin d , cyclin d1 , cyclin b , cyclin dependent kinase , cyclin dependent kinase complex , microbiology and biotechnology , cyclin d3 , cyclin e , biology , chemistry , cancer research , cell cycle , gene , biochemistry
Considerable evidence supports the view that D‐type cyclins play a role in G1‐S progression. We found that cyclin D2 directly interacts with Mel‐18, one of the polycomb group gene products in a yeast two hybrid screen. Further, we have determined the binding domains that are required for interaction between cyclin D2 and Mel‐18. The proline/serine‐rich domain (P/S domain) of Mel‐18 is required to interact with cyclin D2, and the N‐terminal region of cyclin D2 is necessary to interact with Mel‐18. A co‐localization study shows that cyclin D2 and Mel‐18 interact within the nucleus. To determine whether Mel‐18 affects cyclin D2 activity, we blocked Mel‐18 expression using an anti‐sense strand system in cyclin D2 over‐expressing cells. The results indicate that cells with reduced Mel‐18 expression levels show more proliferative activity than the controls. These findings are the first report that Mel‐18 directly interacts with cyclin D2 and may inhibit cyclin D2 activity.