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Lactate utilization in mitochondria prevents Bax cytotoxicity in yeast Kluyveromyces lactis
Author(s) -
Košt’anová-Poliaková Daniela,
Šabová L’udmila
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.026
Subject(s) - kluyveromyces lactis , cytochrome c , mitochondrion , lactate dehydrogenase , kluyveromyces , toxicity , biochemistry , yeast , reactive oxygen species , oxygen toxicity , biology , chemistry , apoptosis , oxygen , enzyme , saccharomyces cerevisiae , organic chemistry
In a search for the physiological conditions able to suppress the disruption of electron transport through the inner mitochondrial membrane induced by Bax, we found that respiratory substrate – lactate completely abolished Bax toxicity in yeast Kluyveromyces lactis . The effect of lactate was dependent on the presence of cytochrome c , as no effect was observed in the cytochrome c null strain. The investigation of lactate effect on markers of Bax toxicity showed that: (i) oxidation of lactate did not affect the decrease in oxygen consumption, but (ii) lactate was able to diminish the generation of reactive oxygen species and simultaneously to suppress Bax‐induced cell death. We show that suppression of Bax lethality in K. lactis can be, in addition to anti‐apoptotic proteins, achieved also by the utilization of lactate in the mitochondria.