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Subcellular distribution of chromogranins A and B in bovine adrenal chromaffin cells
Author(s) -
Huh Yang Hoon,
Bahk Sook Jin,
Ghee Jung Yeon,
Yoo Seung Hyun
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.025
Subject(s) - endoplasmic reticulum , secretory protein , intracellular , microbiology and biotechnology , secretion , granule (geology) , secretory pathway , chromaffin cell , biology , subcellular localization , homeostasis , adrenal medulla , cytoplasm , chemistry , biochemistry , endocrinology , golgi apparatus , catecholamine , paleontology
The major secretory granule proteins chromogranins A (CGA) and B (CGB) have recently been shown to play critical roles in inositol 1,4,5‐trisphosphate‐dependent intracellular Ca 2+ mobilizations. We determined here the subcellular distribution of CGA and CGB based on 3D‐images of chromaffin cells, and found that ∼95% of cellular CGA was present in secretory granules while ∼5% was in the endoplasmic reticulum (ER), whereas ∼57% of cellular CGB was in secretory granules while ∼24% and ∼19% were in the ER and nucleus, respectively. These results suggest that chromogranins are at the center of intracellular Ca 2+ homeostasis in secretory cells.