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Localization of a putative ClC chloride channel in spinach chloroplasts
Author(s) -
Teardo Enrico,
Frare Erica,
Segalla Anna,
Marco Veronica De,
Giacometti Giorgio Mario,
Szabò Ildikò
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.005
Subject(s) - chloroplast , arabidopsis thaliana , chloroplast membrane , spinach , biochemistry , chemistry , chloride channel , arabidopsis , biology , gene , thylakoid , mutant
Seven genes seem to encode for putative ClC chloride channels (AtClC‐a to AtClC‐g) in Arabidopsis thaliana . Their function and localization is still largely unknown. AtClC‐f shares considerable sequence similarity with putative ClC channel proteins from Synechocystis , considered to represent the precursor of chloroplasts. We show by biochemical and mass spectrometry analysis that ClC‐f is located in the outer envelope membrane of spinach chloroplasts. Consistent with the plastidial localization of ClC‐f, p ‐chlorophenoxy‐acetic acid (CPA) reduces photosynthetic activity and the protein is expressed in etioplasts and chloroplasts but not in root tissue. These findings may represent a step toward the molecular identification of ion channel activities in chloroplast membranes.