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Uptake of cell‐penetrating peptides in yeasts
Author(s) -
Holm Tina,
Netzereab Semharai,
Hansen Mats,
Langel Ülo,
Hällbrink Mattias
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.099
Subject(s) - yeast , intracellular , saccharomyces cerevisiae , cell , candida albicans , chemistry , degradation (telecommunications) , cell wall , biochemistry , biophysics , microbiology and biotechnology , biology , telecommunications , computer science
The uptake of different cell‐penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans , was studied using fluorescence HPLC‐analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF) 3 K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studies.