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Enhancement and inhibition of DNA transcriptional activity by spermine: A marked difference between linear and circular templates
Author(s) -
Luckel François,
Kubo Koji,
Tsumoto Kanta,
Yoshikawa Kenichi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.095
Subject(s) - spermine , dna , circular dna , polyamine , template , transcriptional activity , chemistry , molecule , microbiology and biotechnology , biophysics , biochemistry , stereochemistry , biology , gene , genome , gene expression , nanotechnology , enzyme , materials science , organic chemistry
We compared the transcriptional activities of the circular and linear forms of short (4 kbp) and giant (106 kbp) DNA molecules in the presence of a polyamine, spermine (4+). With an increase in the spermine concentration, transcriptional activity was enhanced, followed by an inhibitory effect, and complete inhibition was observed in the sole case of long, linear templates. A difference between the transcriptional properties of circular and linear conformations is found for giant DNA molecules. These results are discussed in relation to DNA conformational transitions.