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Molecular recognition of nucleic acids: Coralyne binds strongly to poly(A)
Author(s) -
Xing Feifei,
Song Guangtao,
Ren Jinsong,
Chaires Jonathan B.,
Qu Xiaogang
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.091
Subject(s) - nucleic acid , chemistry , molecular recognition , biochemistry , biophysics , stereochemistry , biology , organic chemistry , molecule
The small molecule coralyne was found to bind preferentially and strongly to single‐stranded poly(A) with an apparent association constant ( K a ) of (1.8 ± 0.3) × 10 6 M −1 . Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bases. Poly(A) forms a coralyne dependent secondary structure with a melting temperature of 60 °C, for the conditions of our study.