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Coat proteins of two filamentous plant viruses display NTPase activity in vitro
Author(s) -
Rakitina Daria V.,
Kantidze Omar L.,
Leshchiner Anna D.,
Solovyev Andrey G.,
Novikov Viktor K.,
Morozov Sergey Yu.,
Kalinitalia O.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.083
Subject(s) - coat protein , in vitro , coat , chemistry , biology , biochemistry , microbiology and biotechnology , virology , ecology , rna , gene
Coat proteins (CPs) of plant viruses are involved in different stages of the viral life cycle such as virion assembly, replication, movement, vector transmission, and regulation of host defense responses. Here, we report that the CPs of two filamentous RNA viruses, potato virus X (PVX, Potexvirus ) and potato virus A (PVA, Potyvirus ) exhibit an enzyme activity. The CP isolated from PVX virions possesses ATP‐binding and ATPase activities. Recombinant PVX and PVA CPs produced in Escherichia coli show Mg 2+ ‐dependent ATPase and UTPase activities inhibited by antibodies against virus particles. Deletion of the C‐terminal regions of these proteins diminishes their ATPase activity.