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Up‐ and downregulated genes in muscles that undergo developmentally programmed cell death in the insect Manduca sexta
Author(s) -
Löw Péter,
Talián Gábor Csaba,
Sass Miklós
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.079
Subject(s) - manduca sexta , manduca , biology , transcription factor , gene , programmed cell death , gene expression , ecdysteroid , downregulation and upregulation , messenger rna , microbiology and biotechnology , insect , regulation of gene expression , genetics , apoptosis , endocrinology , botany , hormone
This study was designed to investigate changes in gene expression associated with stage‐specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta . The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD in ISMs. Expression of E 75 B transcription factor was repressed while another factor, βFTZ‐F1 , stayed at a very low level. However, gene coding for a translation‐initiation factor ( eIF1A ) was upregulated. Expression of these genes had not been previously reported to be altered in dying ISMs. An ecdysteroid agonist, RH‐5849, that prevented PCD in ISMs also blocked these changes.