Premium
A previously found thylakoid membrane protein of 14 kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI‐P
Author(s) -
Khrouchtchova Anastassia,
Hansson Maria,
Paakkarinen Virpi,
Vain Julia P.,
Zhang Suping,
Jensen Poul Erik,
Scheller Henrik Vibe,
Vener Alexander V.,
Aro Eva-Mari,
Haldrup Anna
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.061
Subject(s) - protein subunit , thylakoid , phosphoprotein , photosystem ii , photosystem i , chemistry , photosystem , biochemistry , biology , biophysics , phosphorylation , chloroplast , photosynthesis , gene
We show that the thylakoid membrane phosphoprotein TMP14 is a novel subunit of plant photosystem I (PSI). Blue native/SDS–PAGE and sucrose gradient fractionation demonstrated the association of the protein exclusively with PSI. We designate the protein PSI‐P. The presence of PSI‐P subunit in Arabidopsis mutants lacking other PSI subunits was analyzed and suggested a location in the proximity of PSI‐L, ‐H and ‐O subunits. The PSI‐P protein was not differentially phosphorylated in state 1 and state 2.