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Histidine inhibits oxidative stress‐ and TNF‐α‐induced interleukin‐8 secretion in intestinal epithelial cells
Author(s) -
Son Dong Ok,
Satsu Hideo,
Shimizu Makoto
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.038
Subject(s) - secretion , histidine , hydrogen peroxide , tumor necrosis factor alpha , microbiology and biotechnology , chemistry , oxidative stress , cytokine , inflammation , biochemistry , biology , amino acid , endocrinology , immunology
We investigated the effect of several amino acids on the secretion of such inflammatory cytokines as interleukin‐8 (IL‐8) induced by hydrogen peroxide or tumor necrosis factor‐alpha (TNF‐α) in intestinal epithelial‐like Caco‐2 and HT‐29 cells. We found that histidine, one of the conditionally essential amino acids, significantly inhibited both hydrogen peroxide‐ and TNF‐α‐induced IL‐8 secretion and mRNA expression in Caco‐2 cells and HT‐29 cells. These inhibitions were dose dependent and the inhibition rate of hydrogen peroxide‐induced IL‐8 secretion reached more than 50% at a concentration of 25 mM, with over 95% inhibition at a concentration of 50 mM. TNF‐α increased the transcriptional activity of the IL‐8 promoter which was significantly inhibited by treating Caco‐2 cells with histidine. Histidine also abolished the NF‐κB‐dependent activation of the IL‐8 promoter induced by TNF‐α. These results indicate that histidine inhibited the hydrogen peroxide‐ and TNF‐α‐induced IL‐8 secretion at the transcriptional level in intestinal epithelial cells, suggesting that histidine has the potential to attenuate intestinal inflammation.