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The yeaS ( leuE ) gene of Escherichia coli encodes an exporter of leucine, and the Lrp protein regulates its expression
Author(s) -
Kutukova Ekaterina A.,
Livshits Vitaliy A.,
Altman Irina P.,
Ptitsyn Leonid R.,
Zyiatdinov Michael H.,
Tokmakova Irina L.,
Zakataeva Natalia P.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.031
Subject(s) - leucine , methionine , amino acid , gene , histidine , biochemistry , escherichia coli , biology , chemistry
Overexpression of the yeaS gene encoding a protein belonging to the RhtB transporter family conferred upon cells resistance to glycyl‐ l ‐leucine, leucine analogues, several amino acids and their analogues. yeaS overexpression promoted leucine and, to a lesser extent, methionine and histidine accumulation by the respective producing strains. Our results indicate that yeaS encodes an exporter of leucine and some other structurally unrelated amino acids. The expression of yeaS (renamed leuE for “leucine export”) was induced by leucine, l ‐α‐amino‐ n ‐butyric acid and, to a lesser extent, by several other amino acids. The global regulator Lrp mediated this induction.