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A transposon‐based activation‐tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations
Author(s) -
Schneider Anja,
Kirch Thomas,
Gigolashvili Tamara,
Mock Hans-Peter,
Sonnewald Uwe,
Simon Rüdiger,
Flügge Ulf-Ingo,
Werr Wolfgang
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.030
Subject(s) - transposable element , mutant , biology , genetics , myb , arabidopsis thaliana , transposon mutagenesis , population , arabidopsis , mutagenesis , transcription factor , microbiology and biotechnology , gene , demography , sociology
A population of 9471 stable activation‐tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize En / Spm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse‐based screen identified six dominant or semi‐dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high‐throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant ( hpc1 ‐ 1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3‐MYB transcription factor.