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Tobamovirus‐resistant tobacco generated by RNA interference directed against host genes
Author(s) -
Asano Momoko,
Satoh Rena,
Mochizuki Atsuko,
Tsuda Shinya,
Yamanaka Takuya,
Nishiguchi Masamichi,
Hirai Katsuyuki,
Meshi Tetsuo,
Naito Satoshi,
Ishikawa Masayuki
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.021
Subject(s) - tobamovirus , tobacco mosaic virus , nicotiana tabacum , biology , gene , genetics , rna , arabidopsis thaliana , rna interference , plant virus , cucumovirus , cucumber mosaic virus , virology , virus , mutant
Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOM1 and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and NtTOM3 in N. tabacum resulted in nearly complete inhibition of the multiplication of Tomato mosaic virus and other tobamoviruses, but did not affect plant growth or the ability of Cucumber mosaic virus to multiply. As TOM1 and TOM3 homologues are present in a variety of plant species, their inhibition via RNA interference should constitute a useful method for generating tobamovirus‐resistant plants.