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Regulation of uncoupling protein activity in phosphorylating potato tuber mitochondria
Author(s) -
Navet Rachel,
Douette Pierre,
Puttine-Marique Florence,
Sluse-Goffart Claudine M.,
Jarmuszkiewicz Wieslawa,
Sluse Francis E.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.07.008
Subject(s) - respiration , mitochondrion , antimycin a , phosphorylation , uncoupling agents , gtp' , chemistry , biochemistry , malonate , biology , botany , enzyme
In isolated potato tuber mitochondria, palmitic acid (PA) can induce a H + leak inhibited by GTP in the phosphorylating (state 3) respiration but not in the resting (state 4) respiration. The PA‐induced H + leak is constant when state 3 respiration is decreased by an inhibition of the succinate uptake with n ‐butyl malonate ( n BM). We show that the efficiency of inhibition by GTP is decreased when state 3 respiration is progressively inhibited by antimycin A (AA) and is restored following subsequent addition of n BM. We propose that in phosphorylating potato tuber mitochondria, the redox state of ubiquinone, which can antagonistically be varied with AA and n BM, modulates inhibition of the PA‐activated UCP‐sustained H + leak by GTP.