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Theoretical model of the interactions between Ca 2+ , calmodulin and myosin light chain kinase
Author(s) -
Fajmut Aleš,
Brumen Milan,
Schuster Stefan
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.076
Subject(s) - myosin light chain kinase , calmodulin , myosin , biophysics , chemistry , phosphorylation , immunoglobulin light chain , kinase , biochemistry , enzyme , biology , antibody , immunology
Active Ca 2+ /calmodulin (CaM)‐dependent myosin light chain kinase (MLCK) plays an important role in the process of MLC phosphorylation and consecutive smooth muscle contraction. Here, we propose a mathematical model of a detailed kinetic scheme describing interactions among Ca 2+ , CaM and MLCK and taking into account eight different aggregates. The main model result is the prediction of the Ca 2+ dependent active form of MLCK, which is in the model taken as proportional to the concentration of Ca 4 CaM · MLCK complex. Wegscheider's condition is additionally applied as a constraint enabling the prediction of some parameter values that have not yet been obtained by experiments.