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Blastocladiella emersonii expresses a centrin similar to Chlamydomonas reinhardtii isoform not found in late‐diverging fungi
Author(s) -
Ribichich Karina Fabiana,
Gomes Suely Lopes
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.075
Subject(s) - chlamydomonas reinhardtii , biology , gene isoform , organelle , chlamydomonas , microbiology and biotechnology , saccharomyces cerevisiae , basal body , cytoplasm , multinucleate , botany , biochemistry , flagellum , yeast , gene , mutant
Centrins are members of the calcium‐binding EF‐hand protein superfamily which can be divided into two subfamilies, probably associated with different functions: one related to Chlamydomonas reinhardtii centrin, CrCenp, and the other, represented by Saccharomyces cerevisiae isoform, ScCdc31p. ESTs encoding the two isoforms (BeCen1 and BeCen3) from the chytridiomycete Blastocladiella emersonii were isolated, and expression of the CrCenp‐type centrin, BeCen1, was analyzed throughout the fungus life cycle. Becen1 mRNA levels increase transiently during sporulation and protein levels present a similar pattern. Immunolocalization studies seem to localize BeCen1 at the basal body zone and in the cytoplasm surrounding the nuclear cap, a zoospore organelle.