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Neisseria meningitidis pili induce type‐IIA phospholipase A 2 expression in alveolar macrophages
Author(s) -
Touqui Lhousseine,
Paya Miguel,
Thouron Françoise,
Guiyoule Annie,
Zarantonelli Maria-Leticia,
Leduc Dominique,
Wu Yongzheng,
Taha Muhamed-Kheir,
Alonso Jean-Michel
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.067
Subject(s) - neisseria meningitidis , pilus , mutant , microbiology and biotechnology , lipopolysaccharide , chemistry , strain (injury) , phospholipase , neisseriaceae , inducer , biology , bacteria , escherichia coli , biochemistry , gene , enzyme , immunology , antibiotics , genetics , anatomy
Induction of type‐IIA secreted phospholipase A 2 (sPLA 2 ‐IIA) expression by bacterial components other than lipopolysaccharide has not been previously investigated. Here, we show that exposure of alveolar macrophages (AM) to Neisseria meningitidis or its lipooligosaccharide (LOS) induced sPLA 2 ‐IIA synthesis. However, N. meningitidis mutant devoid of LOS did not abolish this effect. In addition, a pili‐defective mutant exhibited significantly lower capacity to stimulate sPLA 2 ‐IIA synthesis than the wild‐type strain. Moreover, pili isolated from a LOS‐defective strain induced sPLA 2 ‐IIA expression and nuclear factor kappa B (NF‐κB) activation. These data suggest that pili are potent inducers of sPLA 2 ‐IIA expression by AM, through a NF‐κB‐dependent process.