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Effect of new antioxidant cysteinyl‐flavanol conjugates on skin cancer cells
Author(s) -
Lozano Carles,
Torres Josep Lluís,
Julià Lluís,
Jimenez Aurora,
Centelles Josep Joan,
Cascante Marta
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.051
Subject(s) - dna laddering , chemistry , hacat , apoptosis , catechin , antioxidant , dna fragmentation , biochemistry , polyphenol , programmed cell death , in vitro
Novel catechin derivatives obtained from grape procyanidins and l ‐cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4β‐( S ‐cysteinyl)epicatechin 3‐ O ‐gallate has a free radical scavenging capacity as strong as that of tea (−)‐epigallocatechin gallate and causes a significant S‐phase cell‐cycle arrest in both cell lines at doses higher than 100 μM. The other cysteinyl compounds do not affect normal cell cycle distribution. The gallate derivative also induces apoptosis in melanoma cells more strongly than the other derivatives and the parent (−)‐epicatechin do. The gallate compound seems to trigger nuclear condensation and fragmentation, which is confirmed by DNA laddering. Interestingly, they do not induce apoptosis in keratinocytes (HaCaT).