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Probing cytoskeleton modulation by optical biosensors
Author(s) -
Fang Ye,
Ferrie Ann M.,
Li Guangshan
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.050
Subject(s) - cytoskeleton , biophysics , chinese hamster ovary cell , chemistry , saponin , cytochalasin b , intracellular , biosensor , microbiology and biotechnology , cytochalasin d , cytochalasin , actin cytoskeleton , cell , biology , biochemistry , medicine , receptor , alternative medicine , pathology
This paper reported the use of resonant waveguide grating biosensors for studying the cytoskeleton structure in cells. This was achieved by measuring the changes in mass within the bottom portion of cells upon exposure to saponin in the absence and presence of cytoskeleton modulators. Treatment of Chinese hamster ovary cells with saponin led to a dose‐dependent and dynamic mass changes. When a higher concentration of saponin (>60 μg/ml) was used, a net loss in mass was observed. This is probably resulted from the diffusion of soluble intracellular materials away from the bottom portion of cells after pore formation in the cell plasma membranes by saponin. The pretreatment of cells with actin disruption agents, cytochalasin B and latrunculin A, led to significantly increased loss in cell mass induced by either 75 or 125 μg/ml saponin. These results suggested that optical biosensors provide an attractive means to study the cytoskeleton structure and screen modulators that affect the cytoskeleton structure.